Fig. 5
Effect of treatment with E/M-exo in lung cancer cell lines H1299. a Exosomes derived from 16HBE, A549 and H1299 were taken up by H1299 cells. b Morphology of H1299 cells changed from E- to M- phenotype after M1-, E2- and M2-exo treatment. c The migrated cells numbers were significantly higher in the group with M1- and M2-exo treatment. d More invaded cells were found in E1, M1 and M2-exo treatment groups. e M1-exo induced an increase in N-cadherin and vimentin in H1299 cells. f The mRNA level of N-cad and vimentin and was significantly upregulated after M1- and M2-exosome (100μg/ml) stimulation. (CON-exo, E1-exo, M1-exo, E2-exo, M2-exo represent exosomes derived from 16HBE, E-phenotype A549 cells, M-phenotype A549 cells, E-phenotype H1299 cells, M-phenotype H1299 cells, respectively). (*P < 0.05, **P < 0.01)