Parallel identification of novel antimicrobial peptide sequences from multiple anuran species by targeted DNA sequencing

Table 1 List of primers used for cDNA synthesis and amplification

Target family	Primer	Primer sequence (5′-3′)
Forward primers
Ranidae^a	TP1	CAGGACCAGGGTACGGTGATGTTCACCTTGAAGRAATC
Ranidae^a	TP2	CAGGACCAGGGTACGGTGTTGGGATYGTCTCCTCATCT
Ranidae^a	TP3	CAGGACCAGGGTACGGTGATGTTCACCWTGARKAAAYC
Hylidae^b	TP4	CAGGACCAGGGTACGGTGAAGAARTCWCTTYTTCTTGT
Bombinatoridae^c	TP5	CAGGACCAGGGTACGGTGTAGAAGAAGAATCACTGAGG
Outer PCR Forward	A-Uni1	CCATCTCATCCCTGCGTGTCTCCGACTCAG(X)₁₀CAGGACCAGGGTACGGTG
PCR Reverse	trP1	CCTCTCTATGGGCAGTCGGTGAT

AMP specific sequences are in bold
^aSpecies pertaining to Ranidae family: Pelophylax ridibundus, Pelophylax kl. esculentus, Rana dalmatina, Rana arvalis and Rana temporaria
^bSpecies pertaining to Hylidae family: Hyla arborea
^cSpecies pertaining to Bombinatoridae family: Bombina variegata and Bombina bombina

ISSN: 1471-2164