Parallel identification of novel antimicrobial peptide sequences from multiple anuran species by targeted DNA sequencing

Table 4 Peptides tested for biological activity and their physico-chemical characteristics

Peptide^a	Coding sequence	Sequence^b	MW	Charge	MIC (μM)^c		%Viability^d (100 μM)
					E. coli	S. aureus	Monocytes
#4	rtemp_4.3_210	LVPFIGRTLGGLLARF-NH2	1729.1	+ 4	> 64	4	> 80
#22	rarv_10.1_19	LLGAALSALSSVIPSVISWFQK-NH₂	2286.7	+3	> 64	16	> 80

^aPeptide number refers to designation in Table 3
^bSequences end with a C-terminal amidation signal [51, 52]
^cEvaluated using microdilution assay in MH medium
^dViability assessed in the presence of 100 μM peptide after 24 h exposure by the MTT assay

ISSN: 1471-2164