A transcriptomic snapshot of early molecular communication between Pasteuria penetrans and Meloidogyne incognita

Phani, Victor; Somvanshi, Vishal S.; Shukla, Rohit N.; Davies, Keith G.; Rao, Uma

doi:10.1186/s12864-018-5230-8

Table 1 List of primers used in this study

From: A transcriptomic snapshot of early molecular communication between Pasteuria penetrans and Meloidogyne incognita

S. No.	Transcript name	Primer names	Annotation	Primer sequence (5′ - 3′)	Product length (bp)	Tm (°C)	Purpose
1	TR11426	P1_F	heat shock protein 20	GGAAGAGGAACACAATGGCA	112	60	qRT PCR
1	TR11426	P1_R	heat shock protein 20	TGCCTCAAATTTCCCAGTCC	112	60	qRT PCR
2	TR14120	P3_F	phospholipase A2	ACATTTCTCCATGTCAGCAC	78	60	qRT PCR
2	TR14120	P3_R	phospholipase A2	CGTTGCACTGGAGGAATAAA	78	60	qRT PCR
3	TR16177	P4_F	aspartic protease	CTCCCTATCCTCCACCTATCAA	104	60	qRT PCR
3	TR16177	P4_R	aspartic protease	CACCAAAGCTGACGGTATCA	104	60	qRT PCR
4	TR10194	P5_F	Bm 3887	AAACCTGGCAGATCACAAC	106	60	qRT PCR
4	TR10194	P5_R	Bm 3887	CTCTGCTGTACCACAAACAA	106	60	qRT PCR
5	TR10010	P6_F	fructose bis phosphate aldolase	GACCACCAGATAGGAATACAAC	114	60	qRT PCR
5	TR10010	P6_R	fructose bis phosphate aldolase	GGCAATCTTCACCCAAGAA	114	60	qRT PCR
6	TR31579	P7_F	selenium binding protein	ATATATGAAGGTGGCCCTTGTC	131	60	qRT PCR
6	TR31579	P7_R	selenium binding protein	GCAATTGAAGAACCGACTTCTG	131	60	qRT PCR
7	TR14793	P8_F	glucosyl transferase	CCATTTGACCACTCGATTCA	107	60	qRT PCR
7	TR14793	P8_R	glucosyl transferase	GCATATCGCTCCTCAAATCA	107	60	qRT PCR
8	TR23171	P10_F	venom Allergen like protein	TTGGACGTTGCCCTAGATA	100	60	qRT PCR
8	TR23171	P10_R	venom Allergen like protein	CTACATGGCTCACCAACATT	100	60	qRT PCR
9	TR35213	P11_F	glycoside hydrolase	GGTGATTCCACCAGCATATT	121	60	qRT PCR
9	TR35213	P11_R	glycoside hydrolase	CCAAATGGCCCAGTATCTT	121	60	qRT PCR
10	TR40461	P12_F	glutathione S transferase	TAAGCCAGAAGAGCCGAAA	111	60	qRT PCR
10	TR40461	P12_R	glutathione S transferase	GTGGATCAACTTCGAAAGACTG	111	60	qRT PCR
11	TR11544	P13_F	fatty acid and retinol binding protein	CGAATTGACCGAAGATGACA	106	60	qRT PCR
11	TR11544	P13_R	fatty acid and retinol binding protein	TTCGCTCTTCTCCTTCAATG	106	60	qRT PCR
12	TR26363	P14_F	major sperm protein	ATACGTCGCGGTCTACAA	134	60	qRT PCR
12	TR26363	P14_R	major sperm protein	TTCCGCTTCCGTCCTATT	134	60	qRT PCR
13	TR10990	P15_F	ubiquitin	CCTCGACTGTTCGTGTATTG	101	60	qRT PCR
13	TR10990	P15_R	ubiquitin	GTCATCATCCAACTGACATCC	101	60	qRT PCR
14	TR20164	P17_F	tropomyosin	CGGGCAACCTCATCATATT	108	60	qRT PCR
14	TR20164	P17_R	tropomyosin	GAACCGCTCGTTACAAGAT	108	60	qRT PCR
15	TR24005	P18_F	serine protease	GGGTCATTCGTGCCATTT	108	60	qRT PCR
15	TR24005	P18_R	serine protease	TGGTAATACGACCGTCTACTC	108	60	qRT PCR
16	18S rRNA (HE667742)	18SMiRT F	–	TCAACGTGCTTGTCCTACCCTGAA	155	60	qRT PCR
16	18S rRNA (HE667742)	18SMiRT R	–	TGTGTACAAAGGGCAGGGACGTAA	155	60	qRT PCR
17	GFP (HF675000)	gfp F	–	AGCGGCACGACTTCTTCA	750	60	PCR
17	GFP (HF675000)	gfp R	–	GTGTGGACAGGTAATGGTTGT	750	60	PCR
18	TR10010	FBP_F	fructose bis phosphate aldolase	GCGTCTTCACCTGCATACTT	402	62	PCR
18	TR10010	FBP_R	fructose bis phosphate aldolase	TAAGGCATTGGCAGACCATC	402	62	PCR
19	TR14793	GTfr_F	glucosyl transferase	AGGAATTGCTATTGAGCAGGATA	400	62	PCR
19	TR14793	GTfr_R	glucosyl transferase	GACTGGGACACCAGCATATAAA	400	62	PCR
20	TR26363	Msp_F	major sperm protein	CTTCGCGCTCTTCACTCTT	399	62	PCR
20	TR26363	Msp_R	major sperm protein	CTTCCGCTTCCGTCCTATTC	399	62	PCR
21	TR16177	Asp_F	aspartic protease	CCAGCATCAGATCACGAAGAT	448	62	PCR
21	TR16177	Asp_R	aspartic protease	GGTGGAGGATAGGGAGCTATTA	448	62	PCR
22	TR10990	Ubq_F	ubiquitin	GTTGTCCTAGAGCCAACACTC	400	62	PCR
22	TR10990	Ubq_R	ubiquitin	CGCAATAATGACGATTCGTATGC	400	62	PCR

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ISSN: 1471-2164

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