Fig. 2

Transcriptome analysis of the entire infection process of P. brassicae. a DEGs during the infection stages. The early cortical infection stage (IN) was compared with the RS (resting spores) stage and GS (germinating resting spores) stage. b Significantly enriched Gene Ontology (GO) terms (P < 0.05) of DEGs from the comparison of IN-VS-RS and IN-VS-GS. GO terms belong to biological processes (GOBP), cellular components (GOCC), and molecular functions (GOMF) were shown, respectively. GO terms were sorted based on P-values. c Validation of GO enrichment of DEGs by qRT-PCR. Fifteen DEGs from significant GO Classification Enrichment were chosen randomly for qRT-PCR validation. The relative expression level of each gene was expressed as the fold-change between three different samples (RS, GS, IN) in the RNA-Seq data (red line chart) and qRT-PCR data (black histogram). Data from qRT-PCR represent the means and standard deviations (three replications). The actin gene was used as an internal control to normalize the expression data. Pearson’s correlation coefficient (R-value) was used to measure the consistency of the RNA-seq data and qRT-PCR. DEGs from Biological Process: PlasB_07290, PlasB_01053, PlasB_26969, PlasB_09671; DEGs from Cellular Component: PlasB_02130, PlasB_09605, PlasB_02486, PlasB_01173, PlasB_03329, PlasB_07163; DEGs from Molecular Function: PlasB_09800, PlasB_01922, PlasB_07507, PlasB_01791, PlasB_03590. See Additional file 4: Table S2 for putative functions of these genes. See Additional file 5: Table S3 for genes information