Fig. 3

a Mapping step testing method. Predicted proteins matched by PSTs were compared to the expected proteins, identified by conventional database spectral identifications, using the same spectra and protein sequences database (ORCAE). b Definition of the cluster exon-mapping categories according to their mRNA locations (Ectocarpus sp. GFF3 files, ORCAE): IN when clusters were located inside an mRNA feature, OUT when clusters were located outside an mRNA feature, and CROSS when clusters were located across an mRNA feature. c Clustering step testing method. We only used the green dataset. The number of predicted proteins (matched by IN and CROSS clusters) was compared to that of expected proteins identified with Proteome Discoverer™ using the Ectocarpus sp. database (ORCAE)