Fig. 4

C. elegans ILs constitutively expressing α-synuclein exhibit significantly shorter lifespan. a Survival curves for wild-type (WT) and transgenic nematodes expressing α-synuclein (aS) at 20°C (log-rank test (‘survival’ package, R): N2 p = 0.14; JU1511 p < 0.003; JU1926 p < 4*1e-7; JU1931 p = 0.79 (first 20 days p < 0.0004); JU1941 p < 7e-5, with significant differences between genotypes indicated by stars). b Box-plot of the lifespan for WT and aS lines. Black dots denote outliers (two times interquartile range), with analysis indicating that lifespan is affected by α-synuclein expression, by the genetic background and by the interaction of α-synuclein expression with the genetic background (ANOVA: aS p < 3e-5; Genotype p < < 1e-16; aS x Genotype p < 0.0002; Tukey HSD: N2 p = 0.99; JU1511 p < 0.008; JU1926 p < 0.0004; JU1931 p = 0.71; JU1941 p < 1e-5, with significant differences between genotypes indicated by stars). c Relationship between lifespan and development time. The points represent the mean and the lines indicate the standard error