Table 1 Bacterial strains and plasmids used in this study

Escherichia coli BW25113

Wildtype control for Keio library

Ref. [18]

Escherichia coli ybiA770(del)::kan

ybiA deletion strain

Ref. [18]

Escherichia coli DH5α

Sub-clone host

Ref. [36]

Stenotrophomonas maltophilia D1585

Wildtype host for DLP4

CBCCRRR^a

Stenotrophomonas maltophilia D1585 ΔpilA

Clean deletion of pilA in D1585

Ref. [20]

Stenotrophomonas maltophilia 280

Wildtype host for DLP4

PLPHN/AHS^b

Stenotrophomonas maltophilia 280 ΔpilA

Clean deletion of pilA in 280

Ref. [20]

D1585::DLP4

S. maltophilia D1585 lysogen with DLP4 prophage

This study

pBBR1-MCS

pBBR1MCS broad-host range cloning vector

Ref. [34]

pD1585pilA

pBBR1MCS carrying D1585 pilA, Cm^R

Ref. [20]

p280pilA

pBBR1MCS carrying 280 pilA, Cm^R

Ref. [20]

pYbiA

pBBR1MCS carrying DLP4 ybiA, Cm^R

This study

pFolA

pBBR1MCS carrying DLP4 folA, Cm^R

This study