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Table 8 SNP verified by Sanger sequencing

From: Novel breeding approach for Japanese flounder using atmosphere and room temperature plasma mutagenesis tool

Gene

Locus

Primers 5′-3′

Size (bp)

SNP type

Detected in ARTP treated sample

Detected in control

tp53

9,685,724

F: TGAAGAGCATAGCCAGGAG

228

A > T

m8

/

R: ACCCTGATTCTTTAGGCCT

9,704,973

F: TCAGGTATGGCTTCCTCAC

236

A > G

m5, m6, m8

/

R: AAACAAGACTTACTCGGGAG

9,705,000

F: TCACTCAGGGGAACATGT

192

T > A

m5, m6, m7, m8

/

R: TCAGGATGGTGAGGATCTG

9,705,895

F: TTCCTGTGAGTAATGATGCAGT

226

T > C

m5, m6, m7, m8

/

R: ACATTTGGAATTGATTGCAAAGT

9,712,498

F: CCCATTTGTTCAGAACCGC

164

G > A

m5, m6, m7, m8

/

R: TCCAGTGAGAACGATTGCC

mstn

6,396,324

F: TCTCAGACCGTGATGTTTT

228

C > T

m2, m4, m7

/

R: ATTCCTGGTGGTCTGTG