Fig. 5

Luciferase activity of different truncated Bmotu promoter regulated by BmOVO. a Prediction of cis-acting elements on the promoter of Bmotu (BABH01009636). BmOVO control element (CE) shows as ●, TATA box as ◆, CAAT box as ▍, primer as →, exon as E, the numbers above the line indicates the size of exon or intron, the number below the line indicate the location of exon in genomic sequence (BABH01009636). b Luciferase activity analysis for different truncated Bmotu promoter regulated by BmOVO-1. c Luciferase activity analysis for different truncated Bmotu promoter regulated by BmOVO-2. d Luciferase activity analysis for different truncated Bmotu promoter regulated by BmOVO − 3. BmN cells (10⁵) were transfected with plasmids containing Bmovo expression cassette driven by different truncated otu promoter (1 × 10¹¹ copies) and pRL-TK (1 × 10¹⁰ copies). Luciferase activities in the cells were determined at 60 h post-transfection, 100 μg protein from the lysed cells was used for luciferase assay(*p < 0.05,**p < 0.01, ***p < 0.001)