Fig. 3

Genetic difference analysis data. a An Affymetrix Rat Gene 2.0 ST RNA microarray analysis was performed. Compared to expression in the IRI group, 265 upregulated genes and 267 downregulated genes were detected in the IRI + RPostC group. b-d The differentially expressed ncRNA, mRNA and others were subjected to hierarchical clustering and shown as a heatmap, respectively. The red signal indicates the upregulated genes, and the green signal indicates the downregulated genes. e The horizontal and vertical coordinates of scatter plot represent the log2 value of the expression levels of the two groups, respectively, showing the up-and-down distribution of the genes. f A volcano plot was also created based on the P- and fold-change (FC) values obtained by t-test analysis. The horizontal axis indicates the fold change of the probe, while the vertical axis represents the degree of difference in the probe (−log10 P-value, −LgP)