Fig. 5

Quantitative RT-PCR validation of differentially expressed miRNAs and their corresponding target genes under calcium deficiency and sufficiency. The yellow bar represents relative changes in expression levels, as calculated by the 2^-△△CT method; qRT-PCR analysis was used to analyze the differentially expressed miRNAs. The orange line indicates relative changes in expression levels for differentially expressed miRNAs, as determined by RNA-seq. The blue bars represent changes in relative expression levels of target genes, as determined by qRT-PCR. The purple line indicates changes in relative expression levels of target genes, as calculated by the FPKM using RNA-seq. All qRT-PCRs and RNA-seq analyses were performed for three biological replicates