Fig. 2From: A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasmaRead mapping. a Average proportion of reads mapping to miRNAs (red), other RNA species (blue) and discarded for being too long, short or adapter dimer (grey) in each RNA extraction/library preparation combination (n = 6). b Total reads were downsampled to 5 million and the number of reads mapping to miRNAs was determined for each RNA extraction and library preparation method (n = 6). Error bars show standard error of the mean, significance was determined using Friedman test and Dunn’s multiple comparison test. * p < 0.05 ***p < 0.001, where no p-value shown there was no significance. c Percentages of non-miRNA reads mapping to specific RNA typesBack to article page