Fig. 1

Outline of the feeding trial design during which RFI was measured. During each dietary stage, steers were offered the respective diet for 70 days following a period of dietary adaptation. At the end of each dietary stage, liver biopsies were taken and RFI was calculated. Within breed, all steers were ranked for RFI. RNA-Seq libraries were generated from biopsies taken from the most RFI-divergent steers (n = 12 high and n = 12 low)