Fig. 8From: Genomic reconstruction of σ54 regulons in ClostridialesExperimental validation of the σ54 regulons in Clostridiales species. The EMSAs were performed with the purified SigL (σ54) protein from C. beijerinckii and DNA fragments containing the candidate −24 and − 12 regions upstream of predicted target genes in Clostridiales species. As a negative control (N.C.), the promoter region of CAC2679 gene in C. acetobutylicum was used, which lacks putative −12 and − 24 elementsBack to article page