Fig. 7

Comparison of the Olfr 3’UTR annotations between 4 datasets from adult olfactory mucosa. The male_IS2104, female_IS2014, male_IS2017 and female_IS2017 datasets were analyzed using our IsoSCM pipeline. a Distribution of numbers of 3’UTR isoforms per Olfr. A Fisher-Freeman-Halton exact test shows no significant difference (p = 0.75). b Distribution of sUTR, pUTR and dUTR lengths. The length of sUTRs (left panel) does not appear statistically different between the 4 datasets (two-way ANOVA: experiment effect, F_{(1, 656)} = 2.45, p > 0.05; sex effect, F_{(1, 656)} = 0.88, p > 0.05; no interaction). Whereas the length of pUTRs (middle panel) is significantly lower in the 2017 experiment as compared to the 2014 one (two-way ANOVA: experiment effect, F_{(1, 2253)} = 5.34, p = 0.03; sex effect, F_{(1, 2253)} = 0.18, p > 0.05; no interaction), this difference falls into the 200-nt uncertainty window and may thus not reflect a genuine difference between these 2 experiments. The length of dUTRs (right panel) is statistically different between the 4 datasets (two-way ANOVA: experiment effect, F_{(1, 3319)} = 17.38, p < 0.001; sex effect, F_{(1, 3319)} = 3.06, p > 0.05; no interaction). c Distribution of Olfr genes in 4 distinct quantitative 3’UTR isoform profiles. See Fig. 4 for the definition of the profiles. A Fisher-Freeman-Halton exact test shows no significant difference (p = 0.37)