Fig. 5

Identification and validation of haustorium-specific secreted proteins. a Venn diagram showing the identification of Podosphaera xanthii haustorium-specific secreted proteins. The comparison was carried out using the UniProtKB protein identifiers of complete and annotated secreted proteins deduced from the revised epiphytic and the haustorial transcriptomes of P. xanthii. The box on the right shows the UniProtKB protein ID and unigene name of the P. xanthii haustorium-specific proteins. The asterisk denotes the set of non-annotated proteins detected in the epiphytic secretome. b Detection of transcripts of P. xanthii haustorium-specific secreted proteins in haustorial (Hau) and epiphytic (Epi) structures. Isolation of epiphytic structures and haustorial cells, extraction of RNA and cDNA synthesis were performed as described in Methods. Detection of transcripts was carried out by PCR analysis using the specific primers listed in (Additional file 1: Table S11). The picture shows a representative image of an agarose gel with the results obtained after 3 different PCR experiments with three different RNA samples MW, molecular weight marker HyperLadder 1Kb (Bioline, London, UK)