Fig. 2

RNA-seq analysis of the neonate and adult cervical cord. a, Schematic drawing of the time course of sampling. b, c, Representative images of the injured site of mice selected for RNA-seq. The ventral side of the medulla oblongata of mice injured at P7 (b) or 8 weeks of age (c) are shown. Red indicates PKCγ (a corticospinal tract (CST) marker) signal and blue indicates cell nuclei stained with 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI). White and yellow broken lines indicate the injured and intact side of the CST, respectively. The dorsal side is at the top, ventral is bottom, left is to the left, and right is to the right. Scale bars: 500 μm. A negative control image of (c) is shown in Additional file 1. d, Clustering analysis using the distance matrix of RNA-seq data; n = 3 (lot. 1–3) in each group. The Euclidean distance between two samples is shown in corresponding colors. Blue indicates that the samples were similar (the distance is near) and white indicates that the samples were different (the distance is far)