Fig. 2

Alternative splicing detection and verification in transcripts of N. lugens. a, Distribution of intron boundary types. b, Models for five types of alternative splicing. c, Classification and statistics of alternative splicing events. d, RT-PCR validation of AS events for five genes. The full-length transcript structure of each isoform is shown on the left. Blue boxes and blue lines represent exons and introns, respectively. Orientations from left to right and vice versa indicate that the transcript is located in positive and negative chains, respectively. PCR primers (F, forward and R, reverse) are displayed on the first isoform of each gene. The length of PCR products is shown to the right of the structure of each transcript. Agarose gel electrophoresis bands in the right figure show the PCR result and DNA marker