Fig. 6From: Α de novo 3.8-Mb inversion affecting the EDA and XIST genes in a heterozygous female calf with generalized hypohidrotic ectodermal dysplasiaImmunostaining of the EDA protein in frontal tissue from control (a, b) and HED animals (c, d). Results of the N-ter immunostaining show a stronger contrast between cell wall and cytoplasm coloration in the control animal (a) than in the affected calf (c). Immunostaining of C-ter part of the protein appear similar between control (b) and affected (d) calves. Negative control of the C-ter is available in Additional file 11: Figure S7Back to article page