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Fig. 7 | BMC Genomics

Fig. 7

From: A framework for TRIM21-mediated protein depletion in early mouse embryos: recapitulation of Tead4 null phenotype over three days

Fig. 7

a Representative immunofluorescent signals of TEAD4 and CDX2 in TRIM21-only and TEAD4-depleted embryos at day E4.5 (n = 8 TEAD-depleted and n = 6 TRIM21-only embryos for TEAD4 immunofluorescence; n = 10 TEAD-depleted and n = 6 TRIM21-only embryos for CDX2 immunofluorescence). DNA stained with YO-PRO-1. Size bar, 50 μm. b. Developmental rates during preimplantation. Zygotes injected with mCherry-Trim21 mRNA, n = 212; zygotes injected with mCherry-Trim21 mRNA and anti-TEAD4 antibody, n = 501; zygotes injected with mCherry-Trim21 mRNA and anti-OCT4 antibody, n = 464. c. Developmental rates after blastocyst transfer to uterus. Day E3.5 blastocysts containing mCherry-Trim21 mRNA, n = 30 in 3 recipients; E 3.5 blastocysts containing mCherry-Trim21 mRNA and anti-TEAD4 antibody, n = 30 in 3 recipients; E 3.5 blastocysts containing mCherry-Trim21 mRNA and anti-OCT4 antibody, n = 160 in 20 recipients. Implantation rate: (fetuses + empty decidua + miscarriages) / transferred embryos. Fetal rate: fetuses / transferred embryos (see Methods). Statistical significance tested with Student’s t test. n.s., not significant

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BMC Genomics

ISSN: 1471-2164

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