Table 3 Top sites for modelling IAV virulence based on the 100 models generated from the (A) two-class and (B) three-class IV datasets containing the concatenated aligments of all IAV proteins. For OneR (1R), the numbers in parentheses are the frequency of the corresponding site being selected in the models; for JRip (JR) and PART (PT), they are the average contribution of the corresponding site to accuracy (in percent); and for random forest (RF), they are the average mean decrease in accuracy attributed to the corresponding site. Each number was calculated following 100 independent learnings from the two-class or three-class IV dataset. For 1R, only sites with frequency > 1 are shown, while for JR, PT and RF, only top 20 sites are shown
(A) Two-class IV dataset | |||||
 1R | HA-142 (28) | HA-188 (12) | HA-160 (7) | NA-46 (6) | HA-189 (4) |
PA-X-213 (4) | HA-219 (3) | HA-285 (3) | HA-397 (3) | NA-79 (3) | |
NS1–171 (3) | NS1–95 (3) | HA-196 (2) | NA-86 (2) | NS1–226 (2) | |
 JR | PB2–627 (4.07) | PB2–701 (3.03) | PA-97 (1.40) | HA-297 (1.26) | HA-452 (0.96) |
HA-218 (0.91) | NA-46 (0.89) | M1–227 (0.89) | NA-17 (0.71) | NA-164a (0.58) | |
NS1–95 (0.55) | NS1–226 (0.53) | M1–15 (0.52) | NS1–171 (0.51) | PB2–508 (0.48) | |
NA-151 (0.43) | PA-X-207 (0.43) | NA-29 (0.42) | NA-371 (0.40) | HA-278 (0.39) | |
 PT | NS1–42 (20.29) | PA-97 (20.20) | PB2–714 (18.28) | PB2–110 (16.72) | PB2–153 (13.26) |
PB2–701 (11.53) | NA-276 (10.35) | NP-101 (10.19) | PA-556 (9.94) | PB2–318 (9.26) | |
NP-492 (9.16) | NP-133 (8.92) | PB2–80 (8.71) | M1–215 (8.20) | NS1–123 (7.58) | |
HA-485 (7.56) | PA-341 (6.67) | PB2–635 (6.23) | PB2–158 (6.08) | PB2–627 (5.83) | |
 RF | PA-97 (6.75) | PB2–701 (6.54) | PA-X-97 (6.25) | NS1–42 (5.87) | HA-218 (5.53) |
PB2–355 (5.11) | NP-34 (4.83) | PB2–627 (4.76) | PB2–714 (4.55) | HA-186 (4.12) | |
HA-227 (3.88) | NP-101 (3.78) | PB2–699 (3.68) | HA-485 (3.66) | PB2–318 (3.62) | |
HA-142 (3.52) | M1–30 (3.49) | PB2–675 (3.46) | PB2–153 (3.43) | NA-46 (3.35) | |
(B) Three-class IV dataset | |||||
 1R | HA-188 (34) | NA-370 (16) | NA-16 (10) | HA-142 (9) | HA-53 (6) |
HA-94 (4) | NA-164a (4) | HA-8 (3) | HA-173 (2) | HA-285 (2) | |
 JR | PB2–627 (4.98) | PB2–701 (1.73) | NA-151 (1.45) | NA-164a (1.37) | HA-218 (1.20) |
HA-297 (1.02) | HA-225 (0.94) | HA-452 (0.93) | PB1-F2–28 (0.88) | HA-327b (0.85) | |
M2–28 (0.84) | HA-266 (0.74) | NS1–42 (0.71) | PA-97 (0.68) | NA-61 (0.68) | |
PA-X-213 (0.59) | HA-482 (0.58) | M2–93 (0.54) | HA-160 (0.52) | PB1-F2–49 (0.51) | |
 PT | PB2–158 (12.81) | PB2–110 (11.97) | NS1–42 (10.79) | PB2–153 (10.56) | NA-276 (10.31) |
PB2–80 (9.21) | NS2–67 (8.46) | PB2–265 (8.23) | PB2–66 (7.92) | PB2–627 (7.62) | |
NA-441 (7.28) | NS1–28 (6.97) | M2–24 (6.87) | PB2–497 (6.54) | HA-294 (6.51) | |
PB1–578 (6.20) | PA-97 (6.19) | NP-101 (6.18) | PB2–76 (6.07) | M1–215 (6.06) | |
 RF | PB2–627 (6. 69) | NS1–42 (6.49) | HA-225 (6.41) | PB2–701 (6.34) | PA-97 (5.90) |
HA-218 (5.42) | PB2–355 (5.41) | PA-X-97 (5.26) | M1–215 (4.84) | PB2–699 (4.52) | |
NP-133 (4.51) | NP-101 (4.48) | PB2–153 (4.41) | M1–30 (4.35) | NP-34 (4.31) | |
HA-227 (4.22) | HA-156 (4.17) | PB2–714 (4.12) | HA-188 (4.12) | NA-49 (4.10) | |