Fig. 4
From: Secretory RING finger proteins function as effectors in a grapevine galling insect
Pairwise yeast two-hybrid assay of protein-protein interactions between a D. vitifoliae RING protein (RING-16-700228) and two V. vinifera proteins (CSLD5 and RPS4B). Top: Three fragments of the bait RING protein, the full-length (RING-16-700228-FL) with the signal peptide (SP) domain removed, the RING domain (RING-16-700228-RN), and the C-terminus (RING-16-700228-C), were respectively cloned as baits for the Y2H assay. Bottom: Transformed yeast clones containing both bait and prey plasmids were tested in three dilutions, 200 (0.2 K), 2000 (2 K), and 20,000 (20 K) cells per drop, on DDO and QDOXA medium, respectively. Empty vectors (EV) were used as negative control