Fig. 4
From: miR-380-3p regulates melanogenesis by targeting SOX6 in melanocytes from alpacas (Vicugna pacos)
Effect of miR-380-3p on mRNA and protein expression levels of β-catenin and melanogenic genes. a RT-qPCR analysis of β-catenin, MITF, TYR, TYRP1, and DCT mRNA expression levels in melanocytes transfected with miR-380-3p and its inhibitor compared with those in negative control (NC) melanocytes. b, c Western blot and quantitative analysis of β-catenin, MITF, TYR, TYRP1, and DCT protein levels in melanocytes transfected with miR-380-3p and its inhibitor compared with those in NC melanocytes. mRNA and protein expression levels were normalized to 18S rRNA and β-actin expression, respectively. The bars represent the mean ± standard error (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001