Table 6 Sequences of wheat 1DS and 1DL SSR markers
Marker | Primer sequence (5′-3′) | Annealing temperature (°C) | Arm location | Amplification size (bp) |
|---|---|---|---|---|
wmc147 | F: AGAACGAAAGAAGCGCGCTGAG R: ATGTGTTTCTTATCCTGCGGGC | 58 | 1DS | 154 |
wmc222 | F: AAAGGTGCGTTCATAGAAAATTAGA R:AGAGGTGTTTGAGACTAATTTGGTA | 54 | 1DS | 184 |
gwm337 | F: CCTCTTCCTCCCTCACTTAGC R: TGCTAACTGGCCTTTGCC | 59 | 1DL | 191 |
Xcfd63 | F: TCCTGAGGATGTTGAGGACC R: GAGAGAGGCGAAACATGGAC | 58 | 1DL | 282 |