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Table 4 Number of single nucleotide polymorphism (SNPs) recovered after various filtering stages for five species of shelled pteropods. Hard-filtering was implemented in GATK3.8 VariantFiltration using the following settings: QualByDepth <2.0, FisherStrand >60.0, RMSMappingQuality <5.0, MQRankSumTest <-5.0 and ReadPositionRankSum <-5.0. The hard-filtered SNPs were subsequently filtered to keep those with a minimum site coverage of 5x and present in at least 80% of the individuals. Other filtering options were less stringent, such as a minimum depth of 2x and site presence in at least 50% of individuals

From: Novel genomic resources for shelled pteropods: a draft genome and target capture probes for Limacina bulimoides, tested for cross-species relevance

 

Hard-filtering

80% individuals, 5x depth

80% individuals, 2x depth

50% individuals,5x depth

L. bulimoides

154,864

137,938

137,953

147,763

L. trochiformis

44,014

11,948

12,165

20,518

L. lesueurii

23,379

5359

5847

8487

L. helicina

18,298

2432

2771

4613

H. inflatus

13,041

1371

1559

2092