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Table 1 Genes and protein domains highlighted in this study as a result of functional genome analysis of ten Trichococcus strains. Strains (Locus tag_): 1. T. flocculiformis (Tflo_); 2. Trichococcocus strain ES5 (TES5_); 3. T. pasteurii (Tpas_); 4. T. palustris (Tpal_); 5. T. collinsii (Tcol_); 6. T. patagoniensis (Tpat_); 7. T. ilyis (TR210_); 8. T. alkaliphilus (PXZT_); 9. T. paludicola (Ga019_); 10. T. shcherbakoviae (TART1_)

From: Genome-guided analysis allows the identification of novel physiological traits in Trichococcus species

Feature

Functional genome annotation

Locus tag

Strain

(Protein domains)

1,3-PDO production

 2|3

Glycerol kinase (IPR005999, IPR018483, IPR018484, IPR018485)

TES5_2082|Tpas_2911

 2|3

Dihydroxyacetone activator (IPR009057, IPR015893)

TES5_2083|Tpas_2912

 2|3

Glycerol dehydrogenases (IPR018211, IPR001670)

TES5_2084|Tpas_2913

 2|3

Phosphoenolpyruvate phosphotransferase (IPR004006, IPR004007, IPR012736, IPR004701, IPR012844)

TES5_2085–2087|Tpas_2914–2916

 2|3

1,3-propanediol dehydrogenases (IPR001670, IPR018211)

TES5_2088|Tpas_2917

 2|3

Dihydroxyacetone (IPR004007, IPR012737)

TES5_2089–2090|Tpas_2918–2919

 2|3

Glycerol dehydratase (IPR003206, IPR016176, IPR003208, IPR010254, IPR003207

TES5_2091–2093|Tpas_2920–2922

 2|3

Glycerol dehydratase activator (IPR028975, IPR030994, IPR003208, IPR010254)

TES5_2094–2095|Tpas_2923–2924

 2|3

Cobalamin adenosyltransferase (IPR016030, IPR029499)

TES5_2096|Tpas_2925

 2|3

Hypothetical protein (IPR005624)

TES5_2097,2099|Tpas_2926, 2928

 2|3

Glycerol uptake facilitator (IPR000425, IPR022357, IPR023271)

TES5_2098|Tpas_2927

Alginate utilisation

 5

Alginate lyase (IPR008929)

Tcol_1369,1377,1704

Arabinan utilisation

 6

Glycosyl hydrolase (IPR033132)

Tpat_54,101,590,610,948-949,136,1167,1171,1259,2028,2033,

2527–2528,2577,2585,2682

 6

Metal-dependent hydrolase (IPR032466)

Tpat_57,88,320,321,954,1043,1060,1227,1247,1391, 1392,2241

 6

Extracellular endo-alpha-(1- > 5)-L-arabinanase (IPR032291)

Tpat_1197,1296

Psychrotolerance

 All

Cold-shock protein (IPR000194, IPR003593, IPR004665, IPR011112, IPR011113, IPR011129, IPR012340, IPR027417)

TR210_741|Tpas_88|Tpal_285|TES5_627|Tflo_313| Tcol_65|Tpat_494|PXZT01000007.1_99| Ga0192377_1015_35 | TART1_1674

 All

Cold shock protein signature (IPR002059, IPR011129, IPR012340, IPR019844)

Tpat_494, 1801, 1802, 1901, 1923|Tcol_65, 532, 554, 1698, 1699|Tflo_313, 455, 458, 688, 980|TES5_627, 827, 849, 1357,1359|Tpal_285,869,1036,1801,1820,1877| Tpas_88,599,1471,1472,2297,2758|TR210_741,1024,1470, 1709, 1819, 1842| PXZT01000016.1_53, 1.1_301, 5.1_152, 4.1_46, 5.1_150| Ga0192377_1004_168, 1008_10–11, 1002_82, 1004_145 | TART1_1477, 1504, 2070, 2071, 2352

Salinity tolerance

 7

Glycine betaine transporter OpuD (IPR000060)

TR210_1348

 3|5|6|7|8|9|10

Betaine binding ABC transporter protein (IPR000515)

Tpas_2814–2815|Tcol_1997|Tpat_1468|TR210_2767–2768,2770 | Ga0192364_3215_54–57 | PXZT01000008. _23–26| TART1_2694–96

 1|2|5|7|8|9|10

Osmotically activated choline ABC transporter (IPR003439)

TES5_1206–1209|Tcol_773–776|TR210_342–345|Tflo_1131–1134|Ga0192364_2415_1215| PXZT01000003.2_54–57| TART1_266–69

 2|3|6|7

Choline binding protein A (IPR010126)

TES5_1355|Tpas_1469|Tpat_1570|TR210_2363,1711,2104

 1|2|3|5|6|8|9

Glycine betaine ABC transport system (IPR003439)

TES5_1660–1662|Tcol_1041–1043|Tpas_2619–21|Tpat_203–05|Tflo_1599–01 | Ga0192356_1653_23–25| PXZT01000006.2_75–77

Bacterial defence

 6|10

SNARE associated Golgi protein (IPR032816)

Tpat_1693,1825 | TART1_1950

 4

Tetracycline resistance (IPR004638)

Tpal_1098,1664,1687

 3

Toxin antidote HigA (IPR013430)

Tpas_511

 3

Plasmid system killer (IPR007712)

Tpas_512

 7

Bacteriocin class Iib (IPR010387, IPR029500)

Tflo_874,878–879

 6

Cas9 (IPR028629)

Tpat_1430

 1|2|6

Cas1 (IPR019855)

TES5_196|Tpat_1431|Tflo_184

 6|10

Cas2 (IPR019199)

TES5_195|Tpat_1432| TART1_1189

 1|2|3|7|10

Cas3 (IPR006935)

TES5_201|Tpas_1155|TR210_680|Tflo_179|TART1_0176

 1|2|3|6

Cas5d (IPR021124)

TES5_200|Tpas_1156|TR210_679|Tflo_180

 1|2|6|7

Casd1 (IPR010144)

TES5_199|Tpas_1157|TR210_675|Tflo_181

 1|2|6|7

Csd2 (IPR006482)

TES5_198|Tpas_1158| TR210_677|Tflo_182

 1|2|6|7

Csd4 (IPR022765)

Tpas_1159| TR210_676|Tflo_183