Fig. 4

RT-qPCR of chi-miR-30b-p expression after transfection with mimic and inhibitor. (a) Transfection with 40 nM,80 nM and 120 nM chi-miR-30b-5p mimic in HEK293T cells for 48 h. (b) Transfection with 40 nM, 80 nM and 160 nM chi-miR-30b-5p inhibitor in HEK293T cells for 48 h. (c) Transfection with 40 nM mimic and 80 nM inhibitor in DP cells for 48 h. The expression of chi-miR-30b-p was normalized to U6. Values are mean ± SEM for 3 biological replicates, *P < 0.05, **P < 0.01, ***P < 0.001