Fig. 2

Gene expression profile and GO term enrichment analysis of bone-derived MSCs. a Cumulative number (left) and ratio (right) of single-ended reads generated from the Ion Proton system (1e+ 07 = 10,000,000). b Cumulative number (left) and ratio (right) of sequencing reads aligned to the human reference genome (hg19). Each color represents a different RNA biotype, such as protein-coding RNA, non-coding RNA, and unknown. ‘modified_data’ is Refseq genes expressed in at least one group with FPKM ≥1. c The expression levels of CD14, CD45, CD73, CD90, and CD105 among each type of cells. The RNA-seq data (accession number: SRA245718) of human Macrophage (MΦ) were used as control. d Heatmap showing the relative expression profile of 12,676 genes (FPKM ≥1) among I-MSCs, Mx-MSCs, and Md-MSCs. Scaled expression values are color-coded according to the legend on the left. These genes were categorized into 10 patterns by a hierarchical clustering approach. The genes in each pattern were over-represented by specific GO biological process terms (q <  0.05)