Sequencing Run | Sequencer | Sequencing Kit | Targeted inDrop read depth | Observed inDrop read depth | Mean transcript Quality Score | Mean Barcodes and UMI Quality |
---|---|---|---|---|---|---|
V2 structure mouse 1 | NextSeq | Mid-throughput | 130,000,000 | 148,238,920 | 30.72 | 30.55 |
V2 structure mouse 1 + 10% illumina PhiX | MiSeqa | Nano | 900,000 | 745,903 | 34.94 | 32.24 |
V2 structure mouse 2 and 3 + 15% illumina PhiX | NextSeq | Mid-throughput | 110,500,000b | 122,520,660 | 33.09 | 33.08 |