Table 1 Nucleotide and coding differences identified among the genomes of 86,079/7NS, MS-H and 5 MS-H field isolates
Nucleotide positiona | 86079/7NS | MS-Hb | MS-H3 b | MS-H4 b | MS-H5 b | 101546b | 101731b | Protein/region | Functional category | Amino acid change |
|---|---|---|---|---|---|---|---|---|---|---|
14181 | G | A | A | A | A | A | A | tRNA (Guanine37-N1) methyltransferase | tRNA metabolism | Val→Ile hydrophobic to hydrophobic alkyl to alkyl |
36932 | A | T | T | T | T | T | T | Non-coding region | ||
61685 | G | A | A | A | A | A | A | DNA topoisomerase IV subunit A | Nucleic acid metabolism | Glu→Lys hydrophilic to hydrophilic acidic to basic |
62874 | G | A | A | A | A | A | A | Exonuclease ABC subunit UvrB | Nucleic acid metabolism | Gly→Glu hydrophobic to hydrophilic alkyl to acidic |
67028 | G | A | A | A | A | A | A | tRNA-Trp | tRNA metabolism | |
68587 | T | T | T | C | T | T | T | Hypothetical protein | Unknown | Synonymous substitution |
104704 | G | A | A | A | A | A | A | Non-coding region | ||
107765 | G | A | A | A | A | A | A | ABC transporter protein | Transport | Ala→Val hydrophobic to hydrophobic alkyl to alkyl |
122053 | C | C | C | C | C | T | C | LemA family protein | Unknown | Synonymous substitution |
193771 | G | A | A | G | G | A | A | GTPase ObgE | Environmental sensing | Gly→Arg hydrophobic to hydrophilic alkyl to basic |
194033 | C | C | C | C | C | T | T | GTPase ObgE | Environmental sensing | Ala→Val hydrophobic to hydrophobic alkyl to alkyl |
200404 | T | T | T | A | T | T | T | Hypothetical protein | Unknown | Asp→Val hydrophilic to hydrophobic acidic to alkyl |
201094 | G | A | A | A | A | A | A | P80-related protein | Unknown | Pro→Ser hydrophobic to hydrophilic alkyl to neutral |
203205 | C | A | A | A | A | A | A | Non-coding region | ||
242451 | G | A | A | A | A | A | A | NAD-dependent glyceraldehyde -3-phosphate dehydrogenase | Glucose metabolism | Ala→Val hydrophobic to hydrophobic alkyl to alkyl |
296526 | G | A | A | A | A | G | A | NAD-dependent glyceraldehyde -3-phosphate dehydrogenase | Glucose metabolism | Arg→Lys hydrophilic to hydrophilic basic to basic |
316268 | T | T | T | T | T | T | — | Hypothetical protein | Unknown | Frame Shift |
325032 | — | — | — | — | A | — | — | Hypothetical protein | Unknown | Frame Shift |
325353 | T | T | T | — | T | T | T | Hypothetical protein | Unknown | Frame Shift |
352952 | G | A | A | A | A | A | A | VACB-like ribonuclease II | Nucleic acid metabolism | Ser→Phe hydrophilic to hydrophobic neutral to aromatic |
389629 | G | A | A | A | A | A | A | Aspartate-ammonia ligase | Amino acid metabolism | Ala→Thr hydrophobic to hydrophilic alkyl to neutral |
397783 | T | — | T | T | — | T | T | Peptide ABC transporter ATP-binding protein (OppF) | Transport | Frame Shift |
418984 | A | A | A | T | A | A | A | TatD family deoxyribonuclease | Nucleic acid metabolism | Asp→Val hydrophilic to hydrophobic acidic to alkyl |
433343 | G | A | A | A | A | A | A | Alanine tRNA ligase | tRNA metabolism | Asp→Asn hydrophilic to hydrophilic acidic to neural |
438657 | G | A | A | A | A | A | A | Triacylglycerol lipase | Lipid metabolism | Synonymous substitution |
449402 | G | G | G | A | G | G | G | Cardiolipin synthetase | membrane lipid | Ala→Thr hydrophobic to hydrophilic alkyl to nuetral |
449694 | G | G | G | G | G | G | A | Cardiolipin synthetase | membrane lipid | Ser→Asn hydrophilic to hydrophilic neutral to neutral |
457951 | G | G | G | G | G | G | A | Hypothetical protein | Unknown | Synonymous substitution |
481287 | G | A | A | A | A | A | A | Haemolysin C or DUF21 domain-containing protein | Structural Protein | Ala→Val hydrophobic to hydrophobic alkyl to alkyl |
485166 | C | C | C | C | T | C | C | DHH subfamily | Phosphoesterase function | Synonymous substitution |
498421 | T | C | C | C | C | C | C | Histidyl-tRNA synthetase | tRNA metabolism | Thr→Ala hydrophilic to hydrophobic neutral to alkyl |
502825 | AT | AT | AT | AT | — | AT | AT | Non-coding region | ||
502827 | AT | — | — | AT | — | — | AT | Non-coding region | ||
502829 | — | — | — | AT | — | — | — | Non-coding region | ||
510721 | G | G | G | A | G | G | G | Thymidine phosphorylase | Pyrimidine metabolism | Synonymous substitution |
522899 | G | A | A | A | A | A | A | Hypothetical protein | Unknown | Gly→Glu hydrophobic to hydrophilic alkyl to acidic |
553886 | C | C | C | C | C | T | C | Hypothetical protein | Unknown | Ala→Thr hydrophobic to hydrophilic alkyl to nuetral |
563391 | G | A | A | A | A | A | A | Hexosephosphate transport protein | Transport | Leu→Phe hydrophobic to hydrophobic alkyl to aromatic |
567729 | C | A | A | A | A | A | A | DNA-directed RNA polymerase beta' subunit | Nucleic acid metabolism | Arg→Ile hydrophilic hydrophobic basic to alkyl |
572029 | T | T | T | T | T | T | C | DNA-directed RNA polymerase subunit beta | Nucleic acid metabolism | Glu→Gly hydrophilic to hydrophobic acidic to alkyl |
584838 | G | A | A | A | A | A | A | Hypothetical protein | Unknown | Synonymous substitution |
604569 | C | C | C | C | C | A | C | Hypothetical protein | Unknown | Asp→Glu hydrophilic to hydrophilic acidic acidic |
615741 | G | A | A | A | A | A | A | Potassium uptake protein KtrB | Transport | His→Tyr hydrophilic to hydrophilic basic to neutral |
628272 | G | A | A | A | A | A | A | DNA polymerase III alpha subunit | Nucleic acid metabolism | Ser→Phe hydrophilic to hydrophobic neutral to aromatic |
637130 | G | G | G | G | G | A | G | YbhB/YbcL family Raf kinase inhibitor-like protein | Protease inhibitor | Val→Ile hydrophobic to hydrophobic alkyl alkyl |
651973 | C | C | C | A | C | C | C | S1 RNA-binding domain-containing protein | RNA metabolism | Ser→Ile hydrophilic to hydrophobic neutral to alkyl |
679814 | G | A | A | A | A | A | A | Cation-transporting P-type ATPase | Transport | Synonymous substitution |
686265 | G | A | A | A | A | A | A | Non-coding region | ||
716623 | G | A | A | A | A | A | A | Hypothetical protein | Unknown | Ser→Leu hydrophilic to hydrophobic neutral to alkyl |
737180 | G | A | A | A | A | A | A | Hypothetical protein | Unknown | Synonymous substitution |
780235 | AT | AT | AT | — | AT | AT | AT | Non-coding region | ||
780237 | AT | — | — | — | — | — | — | Non-coding region | ||
794057 | G | A | A | A | A | A | A | Uridine monophosphate kinase | Central metabolism | His→Tyr hydrophilic to hydrophilic basic to neutral |