Fig. 5

The invasion of amplicon sequences on the micro B of A. mexicanus experimentally confirmed using qPCR and FISH. a Coverage plots of apa-sat 26–129 satellite and tnf-8 like gene along with the respective GDR qPCR results comparison between B- and B+ genomes. The higher coverage and GDR in B+ genome indicates the duplicated copies of these sequences on the B chromosome. b FISH mapping further validated these sequences and showed specific marks (red) in the micro B (white arrows). The metaphasic chromosomes are counterstained with DAPI (blue). c Coverage plots of representative B blocks on micro B with corresponding GDR of qPCR. The BLASTn alignments of these representative blocks to Ensemble annotation databases, resulted in several overlapping genes, such as tnf-8 (function: cell death), dpysl2b (function: microtubules binding activity), fgf11b (function: development, morphogenesis, mitogenic and cell survival activities), Zinc finger BED domain daysleeper like (function: chromatin remodelling), zgc:77262 (function: mRNA splicing) ralgps1 (function: cytoskeleton organization) and dchs (function: cell adhesion)