Fig. 1

Differences between the ASV and OTU methods for Shannon diversity and differentially abundant families in a bacterial soil dataset. a Shannon diversity per treatment (treatments 1 and 2) for each method. Samples are displayed as dots (n = 16). Asterisks indicate significant differences between diversity measurements (P < 0.05). b Selected families of the bacterial soil dataset with their respective relative abundance per treatment for the ASV and OTU methods. The presence of dots above the families indicate a significant effect of the applied treatment (FDR 5%) and the dot size corresponds to the logFC. The light and dark blue color marks a decrease or an increase in relative abundance, respectively. The gray boxes are families found to be significant in both methods