Fig. 3

RNA polymerase II second largest subunit (rpb2) haplotype network of 12 haplotypes identified from 100 Fusarium oxysporum and F. oxysporum f. sp. koae isolates including 84 untested, field-collected isolates and 16 characterized isolates of high, moderate, and low virulence and non-pathogenic. All highly virulent isolates clustered in the Hap_3 haplotype. Moderate and low virulence isolates clustered in the Hap_1 haplotype. The characterized non-pathogenic isolates clustered in the Hap_2, Hap_4, and Hap_6 haplotypes. The rpb2 haplotype network is overlaid with amplification from “core” chromosome primer pair P4 (designated by light green) and LSX primer pair P6 (designated in pink). Only the Hap_3 haplotype yielded amplification product with the LSX primer pair P6 from DNA of all highly virulent F. oxysporum f. sp. koae (Fo koae) and six uncharacterized isolates. Every isolate that amplified with the LSX primer pair also amplified with the “core” primer pair. “Core” chromosome primers (P4) produced amplification from three haplotypes including all highly virulent isolates in Hap_3 and two non-pathogenic isolates in Hap_4