Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature

Arshad, Muhammad; Puri, Alpa; Simkovich, Aaron J.; Renaud, Justin; Gruber, Margaret Y.; Marsolais, Frédéric; Hannoufa, Abdelali

doi:10.1186/s12864-020-07161-1

BMC Genomics

Table 2 Identified proteins with differentially altered abundance in miR156 overexpressing genotype (A8) under heat stress relative to the non-stress control A8 plants

From: Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature

Protein IDs	Locus name	Log2 (fold change)	FDR	annotation
^aG7JFK1	MTR_4g130540	−7.85	0.0064	Heat shock 70 kDa protein
A0A072U9J1	MTR_6g452990	−6.82	0.0080	Heat shock protein 81–2
^aA0A072TL89	MTR_0004s051	−6.52	0.0207	Putative small heat shock protein HSP20
^aG7KG40	MTR_5g078040	−4.57	0.0050	Peroxisomal small heat shock protein
G7JGX6	MTR_4g010130	−4.40	0.0157	Sterol regulatory element-binding protein
G7IF74	MTR_1g088640	−4.31	0.0048	Putative universal stress protein A
^aG7IRL3	MTR_2g089340	−3.60	0.0076	Dihydroxyacid dehydratase
G7LGJ8	MTR_8g095680	−3.33	0.0173	Calnexin 2
^aG7KWU8	MTR_7g024390	−3.24	0.0160	Heat shock cognate 70 kDa protein
^aG7L491	MTR_7g012820	−2.70	0.0080	Casein lytic proteinase B3
G7K8X5	MTR_5g059210	−2.63	0.0340	Ubiquitin-fold modifier 1
G7JM88	MTR_4g057200	−2.54	0.0048	Lethal leaf-spot protein, putative
G7K9T0	MTR_5g038460	−2.41	0.0204	Plant/T7N9–9 protein
A0A072UC14	MTR_7g077400	−2.29	0.0058	Acyl-CoA thioesterase
G7JI82	MTR_3g082660	−2.06	0.0076	Bacterial long-chain fatty acid CoA synthetase
^aG7KEN6	MTR_5g097320	−1.89	0.0258	Heat shock protein 81–2
G7IQD5	MTR_2g045050	−1.86	0.0209	Acyl-CoA thioesterase
G7L9N5	MTR_8g089560	−1.76	0.0380	Putative RIN4, pathogenic type III effector
A2Q5W0	MTR_7g085800	−1.68	0.0422	Tubulin alpha chain
^aB7FKA1	MTR_4g021570	−1.58	0.0350	Armadillo/beta-catenin-like repeat protein
G7JNV8	MTR_4g106880	−1.55	0.0069	Peroxisomal membrane PEX14-like protein
G7IMW8	MTR_2g034900	−1.51	0.0076	Importin subunit alpha
G7JNZ5	MTR_4g122670	−1.38	0.0105	Mevalonate/galactokinase family protein
^aA0A072UL44	MTR_4g063710	−1.29	0.0173	Heat shock cognate 70 kDa protein
G7L8K5	MTR_8g046300	−1.20	0.0258	Prohibitin
G7JRF5	MTR_4g036260	−1.19	0.0202	Quinone-oxidoreductase-like protein
G7K595	MTR_5g016590	−1.18	0.0301	Proteasome subunit alpha type
A0A072TUS4	MTR_8g099795	−1.16	0.0292	Heat shock 70 kDa protein
G7I7Q4	MTR_1g025430	−1.15	0.0173	Heat shock protein 81–2
G7I836	MTR_1g082870	−1.10	0.0173	Mitochondrial Rho GTPase
G7LIP6	MTR_8g086070	−1.03	0.0341	Dicarboxylate carrier protein
A0A072VXV5	MTR_1g077480	−1.00	0.0392	Alpha-galactosidase
G7KUS5	MTR_7g022440	1.03	0.0329	Glucose-6-phosphate 1-dehydrogenase
G7JW95	MTR_5g022300	1.07	0.0155	Ferredoxin--NADP reductase, chloroplastic
B7FJJ4	MTR_7g005380	1.12	0.0269	Pyruvate dehydrogenase E1 component subunit
G7JAP0	MTR_3g070100	1.15	0.0337	Putative sedoheptulose-bisphosphatase
G7JI05	MTR_4g131760	1.28	0.0096	Glucose-1-phosphate adenylyltransferase
Q45FF2	MTR_2g017520	1.29	0.0080	Q45FF2_MEDTR Pyridoxal 5-phosphate synthase
A2Q5N9	MTR_7g085490	1.29	0.0155	Galactose mutarotase-like
G7IBQ7	MTR_1g086050	1.31	0.0429	Protein translocase subunit SecA
G7K882	MTR_5g027530	1.31	0.0080	Phosphoribulokinase
Q84UC1	MTR_2g021255	1.32	0.0274	Glutamine synthetase
A0A072V8Q4	MTR_2g046710	1.32	0.0144	S-adenosylmethionine synthase
G7IED1	MTR_1g072260	1.36	0.0221	Putative NAD(P)-binding domain-containing
A0A072UUQ2	MTR_4g045980	1.39	0.0173	Photosystem II biogenesis protein
A0A072ULB0	MTR_4g071880	1.39	0.0203	Fructose-bisphosphate aldolase
A0A072UDY2	MTR_5g004680	1.40	0.0173	Presequence protease
A0A072VPY5	MTR_1g023120	1.41	0.0389	Beta-galactosidase
A0A072V4D0	MTR_3g112420	1.42	0.0392	ATP-dependent protease LA (Lon) domain protein
G7J8Z9	MTR_3g092720	1.44	0.0329	Putative ribosomal protein S30Ae/sigma
G7L028	MTR_7g026340	1.46	0.0114	Glucan endo-1,3-beta-glucosidase-like protein
A0A072VMH0	MTR_1g076570	1.47	0.0173	2-methyl-6-phytylbenzoquinone methyltransferase
A0A072V2V0	MTR_3g498725	1.49	0.0185	ATP-dependent Clp protease ATP-binding subunit
A0A072U1Q8	MTR_7g066120	1.54	0.0188	Fructose-1,6-bisphosphatase
A0A072TGR0	MTR_0151s003	1.55	0.0444	Inositol-1-monophosphatase
A9YWS0	MTR_5g030950	1.56	0.0294	Serine hydroxymethyltransferase
I3S8V0	MTR_7g111860	1.57	0.0050	Putative NAD(P)-binding domain-containing protein
G7LJD5	MTR_8g070530	1.62	0.0105	Phototropin-2 protein
G7LE33	MTR_8g093770	1.65	0.0105	40S ribosomal protein S12
A0A072UYT5	MTR_3g068030	1.68	0.0202	Ribulose bisphosphate carboxylase/oxygenase activase
G7LIX6	MTR_8g018510	1.69	0.0086	Lipoxygenase
A0A072TX52	MTR_8g012565	1.73	0.0050	1-deoxy-D-xylulose 5-phosphate reductoisomerase
^aG7JCT4	MTR_4g095360	1.76	0.0130	Putative tripeptidyl-peptidase II
G7L4Q1	MTR_7g077880	1.76	0.0258	Putative HAD-like domain-containing protein
G7K1Y1	MTR_5g079460	1.80	0.0173	PfkB family carbohydrate kinase
G7KG86	MTR_5g011990	1.82	0.0155	Uncharacterized protein
G7KET9	MTR_5g011220	1.84	0.0080	PGR5-like protein 1A
G7I2N9	MTR_1g073130	1.85	0.0479	Carboxy-terminal processing peptidase-like protein
G7K4T4	MTR_5g096670	1.87	0.0258	Fructose-bisphosphate aldolase
G7LA76	MTR_8g074330	1.88	0.0130	Chitinase (Class Ib) / Hevein
A0A072VNF5	MTR_1g096240	1.90	0.0405	Dihydrolipoamide acetyltransferase
A0A072UL99	MTR_4g071190	1.91	0.0202	Uncharacterized protein
G7JBK8	MTR_3g096290	1.91	0.0317	Cyanobacterial and plant NDH-1 subunit O
G7K999	MTR_5g009010	1.92	0.0117	Putative THUMP domain-containing protein
G7LH37	MTR_8g083210	1.95	0.0302	Aspartokinase-homoserine dehydrogenase
A0A072TEN7	MTR_0380s004	1.98	0.0173	Putative nucleoid-associated protein YbaB/EbfC
A0A072VAN9	MTR_2g090200	1.99	0.0048	Photosystem II Pbs27 protein
A0A072VRL6	MTR_1g107340	2.02	0.0156	Limonoid UDP glucosyltransferase, putative
A0A072UYV0	MTR_4g088615	2.03	0.0076	Putative ribosomal protein S5
I3SSE5	MTR_8g005175	2.04	0.0072	Oxygen-evolving enhancer protein
G7JK55	MTR_4g101750	2.06	0.0290	Elongation factor G, chloroplastic
G7KDR6	MTR_5g030020	2.09	0.0301	Putative nucleotide-binding alpha-beta protein
A0A072TTP2	MTR_8g080230	2.12	0.0166	Lipoxygenase
A0A072TLC8	MTR_0003s056	2.16	0.0340	Carboxypeptidase
G7JBQ7	MTR_3g108040	2.19	0.0080	PsbP domain protein
G7JEX7	MTR_4g068280	2.21	0.0048	Putative trigger factor
G7JZK0	MTR_5g071360	2.25	0.0173	Asparagine synthetase [glutamine-hydrolyzing]
A0A072VDJ3	MTR_2g105480	2.38	0.0130	Putative ATPase, AAA-type, P-loop
A0A072UF41	MTR_5g084030	2.39	0.0144	Indole-3-glycerol phosphate synthase
G7JFL4	MTR_4g130680	2.39	0.0329	ATP phosphoribosyltransferase catalytic subunit
G7KEX7	MTR_5g020640	2.47	0.0050	Glucose-6-phosphate 1-epimerase
G7LAE7	MTR_8g091410	2.51	0.0189	Peptidylprolyl isomerase
^aA0A072UMH4	MTR_4g066170	2.53	0.0033	Lipoxygenase
G7J557	MTR_3g100500	2.70	0.0144	Aspartic proteinase nepenthesin-like protein
G7KPU0	MTR_6g088270	2.70	0.0207	Elongation factor Ts, mitochondrial
G8A394	MTR_3g073860	2.74	0.0290	Acetyl-CoA carboxylase
A0A072U5I5	MTR_7g117430	3.20	0.0064	Eukaryotic aspartyl protease family protein
G7L4R0	MTR_7g088340	3.23	0.0155	Magnesium-protoporphyrin IX monomethyl ester
^aA0A072U496	MTR_7g113480	3.26	0.0050	Xaa-pro aminopeptidase P
A0A072VID5	MTR_1g052535	3.27	0.0270	GTP-binding protein TypA/BipA
A0A072UMH6	MTR_6g085010	3.55	0.0096	Aspartic protease in GUARD CELL-like protein
^aG7J6G6	MTR_3g116110	3.98	0.0110	Photosystem II reaction center PsbP family protein
G7LAD5	MTR_8g091320	4.13	0.0050	Myo-inositol 1-phosphate synthase
^aB7FKA0	MTR_5g035010	5.32	0.0080	Polyketide cyclase/dehydrase and lipid transporter
A0A072VIV9	MTR_1g052165	7.39	0.0219	Esterase D, putative

^aProteins that were common to A8 and EV genotypes under heat shock. All other proteins were unique to A8
FDR False discovery rate

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ISSN: 1471-2164

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