Fig. 2

Distributions of P4HB splice variant expression in RNA-seq from FANTOM5. Splice Junction Tag per Million (SJ TPM) is a unit to count the number of a specific isoform junction normalized by the total number of reads for each RNA-seq dataset. a The graph shows the P4HB splice variant distribution. The circle radius indicates the average expression of all samples in SJ-TPM for each splice variant detected. b Fraction of expression of P4HB splice variants in FANTOM5. c The expression of P4HB-02 and P4HB-021 in three types of cell: primary cell, tissue and cell line. d Representative diagram of P4HB-021 expression in all analyzed samples. The amount of dots is proportional to the relative expression. e Visualization of splicing event of P4HB-021. The blue diagram at the bottom indicates a part of P4HB gene, in reverse direction from exon 1 to exon 4. The black arrows indicate the exon 2, with a splice junction to exon 4 associated to an absence of exon 3. The total number of RNA-seq data was 70 samples