Fig. 3
From: jouvence, a new human snoRNA involved in the control of cell proliferation
The overexpression of jou by lentivirus increases cells proliferation. a Cells number of HCT116 cells transduced with jou-lentivirus, at two different MOI (1 and 10) compared to non-transduced cells (NT) determined by VicellXR. b Same batch of cells than A, but spread and grow in 96 cell-plates and evaluated by luminescence (CellTiter-Glo), which quantify the amount of ATP, and consequently but indirectly, the number of cells. Similarly, the amount of luminescence is increased after 96 h for the two tested MOI. c Expression level (Fold change) of the transduced h-jou (overexpression) compared to non-transduced cells. In HCT116, h-jou is increased by about 11-fold for the MOI-1, and 14-fold for MOI-10. d Cells number of Caco-2 cells transduced with jou-lentivirus (LV), at MOI-10 compared to non-transduced cells (NT) determined by VicellXR. e Same batch of cells than D, but spread and grow in 96 cell-plates and evaluated by luminescence (CellTiter-Glo). f Cells number of RPE-1 cells transduced with jou-lentivirus (LV), at MOI-10 compared to non-transduced cells (NT) determined by VicellXR. g Same batch of cells than F, but spread and grow in 96 cell-plates and evaluated by luminescence (CellTiter-Glo). h Cells number of HUVEC transduced with jou-lentivirus (LV) at MOI-10 and MOI-20 compared to non-transduced cells (NT), spread and grow in 96 cell-plates and evaluated by luminescence (CellTiter-Glo). The amount of luminescence is increased after 96 h. Statistics: For the VicelXR (A-D-F) n = 3, for the Luminescence CellTiter-Glo (B-E-G-H), n = 10. For each figure, two independent experiments have been performed. (p-values: * p < 0,05; ** p < 0,005; *** p < 0,0005). Errors bars represent the mean +/− S.E.M. (p-value were calculated using the one-tail unpaired t-test using GraphPad Prism)