Skip to main content

Table 2 Summary of sRNA sequencing analysis of Azospirillum brasilense Sp245

From: Genome-wide identification of Azospirillum brasilense Sp245 small RNAs responsive to nitrogen starvation and likely involvement in plant-microbe interactions

S.No.

Steps

VN

VC

1

Total number of raw sRNA sequence reads

R1: 27200643

R1: 16557793

R2: 27200643

R2: 16557793

2

Total number of Reads after Adapter removal and Quality filtering at q20

R1: 7731698

R1: 5513335

R2: 7731698

R2: 5513335

3

Mapping percentage with reference genome (Azospirillum brasilense Sp245)

97.07%

97.47%

4

Number of assembled reads (sRNA)

15,614

15,424

5

Assembled sRNAs sorted based on size 50-500 bp

3838

3788

6

Total number of differentially expressed sRNAs in both samples VN & VC (Additional File 2)

3860

7

No. of differentially expressed sRNAs, between 50 and 500 bp length, (Additional File 2)

458

8

No. of significantly differentially expressed sRNAs

pvalue: < 0.05 (with FDR) & sRNAs length between 50 and 500 bp (Additional File 2)

59

9

No. of Downregulated sRNA genes (Table 3)

18

10

No. of Upregulated sRNA genes (Table 3)

41

  1. GenBank Assembly accession used for Bowtie: GCA_000237365.1
  2. VC and VN imply strain Sp245 cells grown in SSM with the full strength of nitrogen and SSM with half strength of nitrogen, respectively