Fig. 7

Methylomic variance of a CpG island within the genome of the NHEJ1 Animal flanking a non-homologous end-joining repair in a CRISPR-mediated edit. A non-homologous end-joining repair of a CRISPR-mediated genomic cut flanking a CpG island (CGI; Chr6:113,076,186–113,077,861; yellow bar) was inserted within the genome of the NHEJ1 Animal, resulting in a methylation pattern of the edited animal (blue squares) that was similar to that of the Control 1 Animal at the same location (red diamonds) localized around the CGI edit site (Fig. 6a) as well as 7000 bp upstream and downstream of the edit (Fig. 6b). The gray band indicates considered biological epigenetic variance (+/− 20% change). The dashed line indicates the protospacer adjacent motif (PAM) location of the targeted CRISPR cut site. The comparison of the percent methylation of the CpG sites observed at the localized CGI region for the CRISPR-edited animal (Fig. 5c) and the unedited control animal (Fig. 5d) demonstrates a lack of variance in methylation pattern as a result of non-homologous end-joining insertion of the 48-bp duplex DNA oligo fragment. Blue squares (■) indicate the percent differences in CpG methylation for NHEJ1 Animal from Control 2 Animal at given chromosome locations. Red diamonds (♦) indicate the percent differences in CpG methylation for Control 1 Animal from Control 2 Animal at given chromosome locations