Abbreviated Name | JAX # | Genomic Modification Details | Mouse Strain |
---|---|---|---|
Control 1 | 005304 | Wild-type parental strain | C57Bl/6NJ |
Control 2 | 000664 | Wild-type parental strain | C57Bl/6 J |
HDR1 | 028551 | CRISPR-mediated HDR insertion using the pRosa-Cas9 targeting vector with an 11-kb insert flanked by Rosa26 locus homology regions, extending 1 kb upstream and 4 kb downstream from the XbaI site within exon 1. The insert contained a codon-optimized Cas9 gene linked to an IRES-GFP. | C57B1/6 J-Gt(ROSA)26Sorem1(CAG-cas9*,-EGFP)Rsky/J |
HDR2 | 028543 | CRISPR-mediated HDR insertion of a 2005-bp donor DNA containing eGFP with a poly A signal in an inverted orientation at the start codon of the Rxfp3 locus. The original insertion vector had homology arms of 1 kb, flanking the insertion sequence. | C57BL/6NJ-Rxfp3em2J/J |
NHEJ1 | 028573 | CRISPR-mediated NHEJ insertion of a 48 base pair duplex DNA oligo with no homology arms into the Rosa26 locus. During endogenous DNA repair, a serendipitous indel of −3 bp and + 6 bp occurred. | C57BL/6 J-Gt(ROSA)26Sorem2Mvw/Mvw |