Fig. 3
From: Intricate genetic variation networks control the adventitious root growth angle in apple
Non-allelic interactions between MdLAZY1, MdIAA1, MdDREB2A, and MdHSFB3 and their effects on adventitious RGA. a Yeast two-hybrid (Y2H) assay showing protein-protein interactions between MdLAZY1 and MdIAA1. b and (g) Joint genotype effect estimations of markers G122/b13 (b) and G122/b13/SNP592 (g). Numbers of the hybrids are presented in parentheses. c Yeast-one-hybrid (Y1H) images showing the interactions of the MdLAZY1 promoter with the proteins MdDREB2A and MdHSFB3. d Transient coexpression analysis of MdLAZY1-pro: LUC interacting with 35S:MdDREB2A or 35S:MdHSFB3. e Y2H assay showing protein-protein interactions between MdDREB2A and MdHSFB3. BD-DREB2A-BC (p) indicates a point mutation of C (SNP592) to T in MdDREB2A-BC. f Transient coexpression analysis of MdLAZY1-pro: LUC interacting with 35S:MdDREB2A and 35S:MdHSFB3. Asterisks represent P < 0.05 by Dunnett’s multiple comparison. h and (i) Proposed model for the genetic variation network regulating RGA. The arrow length indicates the gene expression level. Numbers of the hybrids are presented in parentheses