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Fig. 6 | BMC Genomics

Fig. 6

From: Intricate genetic variation networks control the adventitious root growth angle in apple

Fig. 6

Non-allelic interactions and joint effects of MdNPR5, MdLBD41, and MdbHLH48 natural variants on adventitious RGA in leafy cuttings of hybrids derived from ‘BC’ × ‘M9’ apple rootstock. a Y1H image showing the interaction of the MdNPR5 promoter with MdLBD41 and MdbHLH48. b Transient coexpression assay of MdNPR5-pro: LUC variants interacting with 35S:MdLBD41 variants. c Y2H images showing interactions between variants of MdLBD41 and MdbHLH48. d Transient coexpression assay of MdNPR5-pro: LUC interacting with 35S:MdLBD41 and 35S:MdbHLH48 variants. e Joint genotype effect estimates of markers S1272 (C/T), Z312 (T/G), and S1288 (A/C). Numbers of the hybrids are presented in parentheses. Asterisks represent P < 0.05 by Dunnett’s multiple comparison. f and (g) Proposed model for the genetic variation networks regulating apple rootstock RGA by MdNPR5, MdLBD41, and MdbHLH48. The arrow length indicates the gene expression level. Numbers of the hybrids are presented in parentheses

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