RNA sequencing identifies transcriptional changes in the rabbit larynx in response to low humidity challenge

BMC Genomics

Table 1 List of the ten most significantly upregulated and downregulated genes as identified by RNA-Seq

ENSEMBL ID	Gene symbol	log2 FC	FDR	Biomart Annotation
ENSOCUG00000003548	ECCP ^a	−2.574	0.0121	epithelial chloride channel protein^b
ENSOCUG00000024036	COL6A5	−2.550	0.0121	collagen type VI alpha 5 chain
ENSOCUG00000013994	PLA2G4D	−2.529	0.0121	phospholipase A2 group IVD
ENSOCUG00000010912	KRTDAP	−2.505	0.0121	keratinocyte differentiation associated protein
ENSOCUG00000011842	CRNN	−2.197	0.0121	cornulin
ENSOCUG00000029191	–	−2.072	0.0121	Immunoglobulin lambda variable precursor^c
ENSOCUG00000011037	MYH7	−2.030	0.0121	myosin heavy chain 7
ENSOCUG00000014187	MINAR1	−2.009	0.0212	membrane integral NOTCH2 associated receptor 1
ENSOCUG00000008772	FANK1	−1.905	0.0121	fibronectin type III and ankyrin repeat domains 1
ENSOCUG00000011472	FOXJ1	−1.854	0.0121	forkhead box J1
ENSOCUG00000013331	–	1.469	0.0121	glutathione peroxidase^c
ENSOCUG00000027549	–	1.497	0.0212	immunoglobulin heavy constant IG chain C^c
ENSOCUG00000016426	AGER	1.516	0.0300	advanced glycosylation end-product specific receptor
ENSOCUG00000006499	MGARP	1.566	0.0121	mitochondria localized glutamic acid-rich protein
ENSOCUG00000007106	RAE2	1.689	0.0121	ribonuclease 8
ENSOCUG00000027406	LDHA	1.747	0.0120	lactate dehydrogenase A chain^c
ENSOCUG00000024691	ATPB	1.856	0.0121	ATP synthase subunit B^c
ENSOCUG00000024788		1.941	0.0121	L-lactate dehydrogenase A chain-like
ENSOCUG00000003229	MCP-1	2.226	0.0121	macrophage cationic peptide 1^b
ENSOCUG00000008303	MMP12	2.277	0.0364	matrix metallopeptidase 12^b

The twenty genes listed meet both filtering criteria of FDR < 0.05 and |log2 FC| ≥ 1. Annotations were obtained with Biomart from references to NCBI database information
^aECCP is not a formal gene symbol and is used for the purpose of this study
^bGenes selected for validation by RT-qPCR
^cAnnotation not available through Biomart and was obtained by a search of ENSEMBL database by ID. Negative and positive values of log2 FC denote down- and upregulated genes, respectively

ISSN: 1471-2164