Fig. 4

Gene structures of Omp-1/P28 family outer membrane proteins. E. chaffeensis Arkansas encodes 22 copies of Omp-1/P28 major outer membrane proteins clustered in tandem. Ehrlichia sp. HF encodes 23 copies, which are named Omp-1.1 to Omp-1.23 consecutively. However, it lacks homologs to E. chaffeensis Omp-1Z, C, D, F, and P28-2, but has duplicated Omp-1H and 6 copies of Omp-1E (based on best Blastp matches to E. chaffeensis Omp-1/P28 proteins). Note: omp-1.1 of Ehrlichia sp. HF (EHF_0067, ortholog of E. chaffeensis omp-1m) was initially annotated as a pseudogene by NCBI automated annotation pipeline. New start site was determined based on homolog to E. chaffeensis omp-1m. Grey bars indicate non-omp-1 genes within Ehrlichia omp-1/p28 gene clusters