Table 3 Base composition and skewness of the mitogenomes in C. macrostigma and other five representative species of Cobitinae

all mtDNA

16,636

29.5

28.8

25.1

16.6

58.3

41.7

0.013

−0.205

all mtDNA

16,561

31.1

25.6

27.3

16.0

56.7

43.3

0.096

− 0.260

PCGs

11,472

27.3

31.5

25.4

15.9

58.8

41.2

−0.038

− 0.266

PCGs

11,425

28.6

28.1

27.8

15.6

56.6

43.4

0.009

− 0.281

1st of PCGs

3814

26.2

23.2

24.7

25.9

49.4

50.6

0.063

0.025

1st of PCGs

3798

29.1

20.2

27.0

23.7

49.3

50.7

0.179

− 0.064

2nd of PCGs

3814

18.2

40.9

27.3

13.6

59.1

40.9

−0.385

−0.335

2nd of PCGs

3798

19.5

38.9

27.8

13.8

58.4

41.6

−0.331

− 0.337

3rd of PCGs

3814

37.3

30.2

24.3

8.2

67.5

32.5

0.105

−0.496

3rd of PCGs

3798

40.9

20.9

31.4

6.8

61.8

38.2

0.323

−0.644

tRNAs

1557

28.3

26.9

21.6

23.2

55.2

44.8

0.024

0.037

tRNAs

1558

28.6

26.2

22.3

22.9

54.8

45.2

0.044

0.014

rRNAs

2627

33.2

22.1

23.0

21.7

55.3

44.7

0.201

−0.028

rRNAs

2628

35.0

19.3

25.0

20.7

54.3

45.7

0.290

−0.095

CR

917

34.4

32.0

19.4

14.3

66.3

33.7

0.036

−0.152

CR

901

35.6

32.7

18.9

12.8

68.4

31.6

0.042

−0.193

all mtDNA

16,636

29.8

28.0

25.9

16.4

57.7

42.3

0.032

−0.226

all mtDNA

16,570

29.2

27.4

26.5

17.0

56.6

43.4

0.031

−0.219

PCGs

11,471

27.4

30.6

26.3

15.8

58.0

42.0

−0.055

−0.251

PCGs

11,433

26.6

29.9

27.2

16.4

56.4

43.6

−0.059

− 0.247

1st of PCGs

3814

26.1

22.9

24.9

26.1

49.0

51.0

0.065

0.022

1st of PCGs

3800

26.7

21.5

26.8

25.1

48.2

51.8

0.107

−0.032

2nd of PCGs

3814

18.3

40.8

27.3

13.5

59.2

40.8

−0.381

− 0.339

2nd of PCGs

3800

19.7

39.2

27.4

13.6

58.9

41.1

−0.332

−0.336

3rd of PCGs

3814

37.6

27.8

26.8

7.8

65.4

34.6

0.149

−0.550

3rd of PCGs

3800

36.2

25.7

29.7

8.4

61.9

38.1

0.170

−0.557

tRNAs

1563

28.3

26.7

21.7

23.2

55.1

44.9

0.029

0.034

tRNAs

1559

28.3

26.5

22.1

23.1

54.8

45.2

0.033

0.021

rRNAs

2628

34.1

21.9

22.9

21.1

56.0

44.0

0.219

−0.042

rRNAs

2631

33.8

21.2

23.7

21.3

55.0

45.0

0.229

−0.052

CR

916

34.3

31.2

19.9

14.6

65.5

34.5

0.047

−0.152

CR

913

36.0

31.3

18.4

14.2

67.4

32.6

0.070

−0.128

all mtDNA

16,602

30.1

25.4

28.0

16.4

55.6

44.4

0.084

−0.261

all mtDNA

16,566

29.3

27.8

26.7

16.2

57.1

42.9

0.026

−0.244

PCGs

11,432

27.6

27.7

28.7

16.0

55.3

44.7

−0.002

− 0.283

PCGs

11,427

26.9

30.4

27.2

15.6

57.3

42.7

−0.062

−0.270

1st of PCGs

3800

26.0

21.2

26.5

26.3

47.2

52.8

0.103

−0.005

1st of PCGs

3800

25.8

22.5

25.5

26.2

48.3

51.7

0.068

0.013

2nd of PCGs

3800

18.3

40.5

27.6

13.6

58.8

41.2

−0.376

−0.342

2nd of PCGs

3800

18.1

40.5

27.8

13.6

58.6

41.4

−0.382

−0.342

3rd of PCGs

3800

38.2

21.3

32.1

8.4

59.6

40.4

0.283

−0.586

3rd of PCGs

3800

36.5

28.1

28.4

7.1

64.6

35.4

0.131

−0.602

tRNAs

1559

27.8

27.1

21.4

23.7

54.8

45.2

0.013

0.051

tRNAs

1559

27.6

27.5

21.3

23.6

55.1

44.9

0.001

0.051

rRNAs

2635

33.7

19.4

25.4

21.4

53.2

46.8

0.269

−0.084

rRNAs

2623

33.6

21.5

24.1

20.8

55.1

44.9

0.221

−0.075

CR

928

35.0

32.0

19.7

13.3

67.0

33.0

0.045

−0.196

CR

920

32.5

31.5

21.4

14.6

64.0

36.0

0.015

−0.190