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Table 1 Genes differentially trnascribed on plates or in liquid

From: Changes in the expression of genes encoding type IV pili-associated proteins are seen when Clostridium perfringens is grown in liquid or on surfaces

Gene Name

Gene Product

Locus tag

DGE (LFC)a

FDR (q value) a

Genes transcribed at higher levels on plates than liquid

fus

Elongation factor G

CPE0079

2.010

0.0276

mdh

Alcohol dehydrogenase

CPE0085

5.323

0.0405

iolB

Myo-inositol catabolism protein

CPE0088

5.361

0.0276

iolD

Myo-inositol catabolism protein

CPE0089

5.840

0.0276

mviM

Probable dehydrogenase

CPE0090

6.458

0.0405

iolE

Myo-inositol catabolism protein

CPE0091

6.367

0.0405

 

Na+/myo-inositol cotransporter

CPE0092

6.517

0.0405

mviM

Probable dehydrogenase

CPE0093

6.825

0.0405

caiD

Crotonase

CPE0095

6.801

0.0405

 

Propionate CoA-transferase

CPE0096

6.666

0.0405

acdS

Acyl-CoA dehydrogenase

CPE0097

6.615

0.0276

gldA

Glycerol dehydrogenase

CPE0099

3.575

0.0405

ldh

L-lactate dehydrogenase

CPE0103

2.347

0.0276

 

Hypothetical protein

CPE0105

2.200

0.0276

 

Hypothetical protein

CPE0113

4.346

0.0405

 

Probable transposase

CPE0139

2.209

0.0405

rbsK

2-keto-3-deoxygluconate kinase

CPE0146

3.584

0.0405

bglR

Beta-glucuronidase

CPE0147

2.973

0.0276

fabG

Probable oxidoreductase

CPE0149

4.098

0.0276

eda

KDPG/KHG aldolase

CPE0150

4.529

0.0276

uxuA

D-mannonate dehydrolase

CPE0151

4.598

0.0276

uxaC

Glucuronate isomerase

CPE0152

4.107

0.0276

uidB

Glucuronide permease

CPE0153

3.929

0.0508

bglX

Probable beta-hexosamidase A

CPE0154

3.756

0.0276

potE

Membrane-spanning transporter protein

CPE0166

5.109

0.0276

pbg

Beta-galactosidase

CPE0167

6.142

0.0276

arcA

Arginine deiminase

CPE0168

8.558

0.0276

arcB

Ornithine carbamoyl transferase

CPE0169

9.532

0.0276

arcD

Arginine ornithine antiporter

CPE0170

9.285

0.0405

arcC

Carbamate kinase

CPE0171

10.053

0.0276

argR

Arginine repressor

CPE0172

9.292

0.0276

ackA

Acetate kinase

CPE0217

2.473

0.0276

galE

UDP-glucose 4-epimerase

CPE0286

2.507

0.0276

lldP

Probable lactate permease

CPE0310

4.960

0.0405

etfB

Electron transfer flavoprotein beta-subunit

CPE0311

5.305

0.0405

etfA

Electron transfer flavoprotein alpha-subunit

CPE0312

5.131

0.0405

glcD

Probable glycolate oxidase subunit

CPE0313

5.530

0.0405

fucK

Rhamnulokinase

CPE0317

4.832

0.0405

fucI

L-fucose isomerase

CPE0318

5.490

0.0405

fucA

L-fuculose-phosphate aldolase

CPE0319

5.211

0.0405

manX

Phosphotransferase system, mannose/fructose-specific component IIA

CPE0320

5.914

0.0405

manY

Probable PTS system

CPE0322

5.990

0.0405

manZ

Probable PTS system

CPE0323

5.472

0.0276

 

Probable glycosyl hydrolase

CPE0324

4.220

0.0405

 

Hypothetical protein

CPE0325

4.582

0.0276

lacA

Galactose-6-phosphate isomerase

CPE0326

3.756

0.0276

lacB

Galactose-6-phosphate isomerase

CPE0327

4.059

0.0405

 

Hypothetical protein

CPE0329

3.447

0.0405

mgtA

Probable cation-transporting ATPase

CPE0333

2.606

0.0276

ugpB

ABC-type sugar transport system, periplasmic component

CPE0371

3.351

0.0405

ugpA

Probable ABC transporter

CPE0372

3.083

0.0405

ugpE

Probable ABC transporter

CPE0373

3.126

0.0405

aga

Alpha-galactosidase

CPE0374

3.251

0.0276

skn1

Endo-beta-galactosidase C

CPE0375

3.511

0.0276

arcD

Probable amino acid permease

CPE0389

3.325

0.0276

dchS

Histidine decarboxylase

CPE0390

3.404

0.0276

 

Conserved hypothetical protein, six-hairpin glycosidase

CPE0426

3.005

0.0276

dedA

Alkaline phosphatase-like protein

CPE0455

4.214

0.0405

 

Conserved hypothetical protein

CPE0456

5.954

0.0405

nanJ

Exo-alpha-sialidase

CPE0553

3.651

0.0405

 

Hypothetical protein

CPE0554

4.438

0.0405

 

Hypothetical protein, in sigV operon

CPE0560

3.806

0.0405

ribB

Riboflavin biosynthesis protein

CPE0566

4.731

0.0405

pflA

Pyruvate-formate lyase-activating enzyme

CPE0660

2.014

0.0276

 

Hypothetical protein

CPE0669

2.475

0.0276

nanI

Exo-alpha-sialidase

CPE0725

2.373

0.0276

lipB

Probable lipase

CPE0742

2.368

0.0276

cstA

Probable carbon starvation protein (peptide utilization)

CPE0743

2.902

0.0276

fepG

Probable iron (III) dicitrate ABC transporter

CPE0794

2.994

0.0405

 

Conserved hypothetical protein

CPE0806

2.526

0.0276

 

Hypothetical protein

CPE0808

2.776

0.0276

 

Endo-beta-N-acetylglucosaminidase

CPE0818

4.763

0.0405

rubY

Rubrerythrin

CPE0855

5.144

0.0276

ams1

Alpha-mannosidase

CPE0856

2.716

0.0276

gntT

Probable gluconate permease

CPE0860

2.095

0.0498

 

Hypothetical protein

CPE0863

2.281

0.0276

 

Hypothetical protein

CPE0876

2.380

0.0276

pduL

Probable propanediol utilization protein

CPE0905

4.429

0.0276

nrdD

Probable anaerobic ribonucleotide reductase

CPE0917

3.741

0.0276

 

Hypothetical protein

CPE0918

3.713

0.0405

 

Hypothetical protein

CPE0919

2.303

0.0405

pduC

Coenzyme B12-dependent glycerol dehydrogenase large subunit

CPE0929

4.916

0.0405

 

Coenzyme B12-dependent glycerol dehydrogenase medium subunit

CPE0930

5.177

0.0276

pduE

Coenzyme B12-dependent glycerol dehydrogenase small subunit

CPE0931

4.927

0.0276

 

Probable glycerol dehydratase large subunit

CPE0932

5.155

0.0276

 

Conserved hypothetical protein

CPE0934

4.864

0.0405

 

Hypothetical protein

CPE0982

3.345

0.0276

sdhB

L-serine dehydratase beta subunit

CPE0988

2.614

0.0405

sdhA

L-serine dehydratase alpha subunit

CPE0989

2.606

0.0276

tdcF

Probable translation initiation inhibitor

CPE1012

2.954

0.0405

thd

Threonine dehydratase

CPE1165

2.659

0.0276

gltP

Probable glutamate/ aspartate transporter

CPE1167

4.116

0.0276

pfk

6-phosphofructokinase

CPE1185

2.492

0.0276

ugpB

ABC-type sugar transport system, periplasmic component

CPE1257

5.358

0.0405

eno

Enolase

CPE1299

2.171

0.0405

galM

Aldose 1-epimerase

CPE1344

3.795

0.0276

galK

Galactokinase

CPE1345

3.935

0.0405

galT

Galactose-1-phosphate-uridyl transferase

CPE1346

5.420

0.0405

ams1

Alpha-mannosidase

CPE1415

2.732

0.0276

clpB

ATPase with chaperonin activity

CPE1428

2.641

0.0276

 

Uncharacterized membrane protein, peptidase

CPE1452

2.064

0.0276

 

Hypothetical protein

CPE1592

2.340

0.0276

purR

Probable transcriptional regulator, LacI/PurR family

CPE1626

3.087

0.0276

rbsB

Probable ribose ABC transporter

CPE1627

3.103

0.0276

rbsC

Probable ribose ABC transporter

CPE1629

2.619

0.0405

rbsA

Probable ribose ABC transporter

CPE1630

2.403

0.0276

trmU

Probable tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase

CPE1783

2.530

0.0276

nifU

Probable nitrogen fixation protein

CPE1784

2.309

0.0276

dacF

Serine-type D-Ala-D-Ala carboxypeptidase

CPE1806

2.596

0.0276

deoA

Pyrimidine-nucleoside phosphorylase

CPE1807

2.672

0.0276

 

Alpha-L-fucosidase

CPE1876

3.336

0.0405

hipB

Predicted transcriptional regulator

CPE1967

3.060

0.0405

potB

Probable spermidine/putrescine ABC transporter

CPE1969

2.143

0.0405

pldB

Lysophospholipase

CPE1989

2.345

0.0276

podK

Pyruvate phosphate dikinase

CPE2011

2.691

0.0276

potE

Probable glutamate gamma-aminobutyrate antiporter

CPE2060

2.994

0.0405

 

Alpha-glucosidase

CPE2076

2.114

0.0405

nagC

Probable transcriptional regulator

CPE2077

2.213

0.0405

ugpB

ABC-type sugar transport system, periplasmic component

CPE2078

3.148

0.0405

ugpE

Probable ABC transporter

CPE2081

3.132

0.0405

lplB

Probable ABC transporter

CPE2082

3.087

0.0405

glnQ

Probable amino acid ABC transporter

CPE2092

2.263

0.0276

 

Hypothetical protein

CPE2100

2.371

0.0276

 

Probable mercuric ion-binding protein

CPE2151

2.128

0.0276

lraI

Metal transport and potential adhesin

CPE2158

2.531

0.0276

nlpD

Membrane protein related to metalloendopeptidase

CPE2182

2.322

0.0480

lepW

Signal peptidase type I

CPE2295

2.761

0.0405

add

Probable adenosine deaminase

CPE2506

3.538

0.0276

 

Conserved hypothetical protein with CXXC pairs

CPE2549

4.434

0.0276

lpd

Probable oxidoreductase

CPE2550

3.875

0.0276

glpA

Glycerol-3-phosphate dehydrogenase

CPE2551

2.652

0.0276

glpP

Probable glycerol uptake operon antiterminator

CPE2553

2.101

0.0276

agaS

Probable tagatose-6-phosphate aldose/ketose isomerase

CPE2625

2.114

0.0405

gatY

Probable tagatose-bisphosphate aldolase

CPE2626

2.311

0.0276

manY

Probable PTS system

CPE2631

2.527

0.0405

Genes transcribed at higher levels in liquid than on plates

 

Predicted membrane protein in amiC operon

CPE0117

2.138

0.0276

mscL

Large-conductance mechanosensitive channel

CPE0174

2.349

0.0276

adh

Alcohol dehydrogenase

CPE0449

3.342

0.0276

opuBA

Glycine betaine/carnitine/choline ABC transporter

CPE0557

3.320

0.0276

hisJ

Amino acid ABC transporter

CPE0600

2.705

0.0502

hisM

Amino acid ABC transporter

CPE0601

2.959

0.0405

glnQ

Amino acid ABC transporter

CPE0602

3.759

0.0276

argG

Argininosuccinate synthase

CPE0691

3.140

0.0405

 

Hypothetical protein, alone in operon

CPE0768

2.107

0.0276

pfs

5′-methylthioadenosine/S-adenosylhomocysteine nuclosidase

CPE1050

4.068

0.0276

cfa

Cyclopropane-fatty-acyl-phospholipid synthase

CPE1051

2.711

0.0276

sseA

Thiosulfate sulfur transferase

CPE1052

2.314

0.0276

adeC

Adenine deaminase

CPE1268

2.032

0.0276

potD

Spermidine/putrescine-binding protein

CPE1269

2.464

0.0276

nupC

Pyrimidine nucleoside transporter

CPE1284

3.637

0.0276

 

Hypothetical protein, alone in operon

CPE1539

3.105

0.0276

 

Conserved hypothetical protein

CPE1655

2.296

0.0405

 

Probable xanthine/uracil/vitamin C permease

CPE1751

2.101

0.0276

abrB

Stage V sporulation protein T, transcritional regulator

CPE2482

3.013

0.0276

adhE

Aldehyde-alcohol dehydrogenase E

CPE2531

2.187

0.0276

spmB

Spore maturation protein B

CPE2532

2.263

0.0405

glnA

Glutamine synthetase

CPE2569

2.216

0.0405

 

Conserved hypothetical protein, alone in operon

CPE2585

3.485

0.0276

  1. a. DGE (LFC), differential gene expression (log fold change). Calculated from the TPM from 6 samples grown on plates versus 6 samples grown in liquid