Fig. 5

Functional validation of the miR-147b target sites in vg. a Predicted sites of miR-147b in the ORF of vg. The nucleotides in bold italics were mutated for TATACAGT in the PmirGLO-miR-147b-mut target. b Relative expression levels of miR-147b and vg in the body walls of third wingless (3rdWL) and winged (3rdWD) lines. c Luciferase reporter assays were performed by co-transfecting the miR-147b agomir with a luciferase reporter gene linked to the vg targets. Different letters on the histogram bars indicate significant differences based on one-way ANOVA followed by Tukey’s multiple comparison (P < 0.05). Asterisks indicate significant difference based on Student’s t-test (P < 0.05)