3’Pool-seq | TruSeq | Plate-Seq | DRUG-seq | |
---|---|---|---|---|
Library prep cost per sample | $3 | $60 | $3 | $0.2–1 |
Sequencing cost per sample | $12 | $100 | $12* | $2–4* |
Overall time for library prep | 8–12 h | 2–3 days | > 2 days | N/A |
Hands-on time | 2–3 h | 6–8 h | N/A | N/A |
Samples per Run | 96 | 12–24 | 96 | 384–1536 |
Major Advantage | No custom equipment or sequencing primers, stringently benchmarked against ERCC and TruSeq. | Best option for detecting low-abundance genes or splice variants. | Oligo-dT Plate-based RNA purification. | Most affordable option, highest throughput, manual alternative is described. |
Major Disadvantage | Involves RNA purification step, lowest throughput of three 3′-end techniques described herein. | Most expensive option, low-throughput, technically tedious. | Requires custom liquid dispensing equipment, no detailed benchmarking with ERCC or TruSeq. | Requires custom liquid dispensing equipment, manual protocol not benchmarked. |