Fig. 8From: A transcriptional landscape of 28 porcine tissues obtained by super deepSAGE sequencingFlowchart of Super deepSAGE library construction. There are three major steps included in the protocol: a) reverse transcription with oligo (dT) coupled magnetic beads, synthesis of the secondary chain, and digestion with NlaIII; b) add 5′ end linker and digest with EcoP15I, and c) add 3′ end linker and PCR amplification. For details see materials and methods sectionBack to article page