Fig. 3

Hypertonic stress limits gene expression of miRNA biogenesis factors in gill filament. Gill filaments were subjected to different time-point of hypertonic treatment in the differential gene expression analysis. Expression of miRNA biogenesis key factors, drosha, exportin 5 (xpo5), dicer1 and argonaute 2 (ago2), was determined in control (Ctrl: 300 mOsmol l^− 1) or hypertonic-treated (Hyper: 600 mOsmol l^− 1) gill filaments for 4, 8 and 24 h by qRT-PCR. Gapdh was used as internal control. Results (mean ± s.e.m.) were from five independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001 vs control